A device screening method for predicting delivery of inhaled mRNA via a nebuliser

Authors: Imco Sibum, Bernhard Müllinger, Nicolas Buchmann

Event: DDL 2023, 6 – 8 December 2023, Edinburgh, United Kingdom

Abstract: A device screening method is presented which takes aerosol parameters, deposition modelling, and post aerosolisation mRNA integrity data to predict lung deposition of functional mRNA as a percentage of fill dose. In the prediction performed here, the Aerogen Solo, Pari eFlow rapid, and the Resyca Pulmospray™ result in 12%, 2% and 51% lung deposition of functional mRNA, respectively.

The delivery of full-length mRNA is important, as only full-length mRNA results in the translation of the wanted biological active protein. When selecting a drug delivery device for the inhalation of sensitive biologics, such as mRNA formulations, the device specific drug delivery characteristics must be considered.

Based on nebuliser fill volume, large differences in active mRNA delivery efficiently may exist and careful selection of the device is recommended. Particularly for biological compounds that carry high costs, substantial cost savings may be realised when selecting an efficient delivery device. The Resyca Pulmospray™ device is such an efficient device that may provide up to 50% of the filled mRNA to the deep lung intact.